Structure-based membrane dome mechanism for Piezo mechanosensitivity

نویسندگان

  • Yusong R Guo
  • Roderick MacKinnon
چکیده

Mechanosensitive ion channels convert external mechanical stimuli into electrochemical signals for critical processes including touch sensation, balance, and cardiovascular regulation. The best understood mechanosensitive channel, MscL, opens a wide pore, which accounts for mechanosensitive gating due to in-plane area expansion. Eukaryotic Piezo channels have a narrow pore and therefore must capture mechanical forces to control gating in another way. We present a cryo-EM structure of mouse Piezo1 in a closed conformation at 3.7Å-resolution. The channel is a triskelion with arms consisting of repeated arrays of 4-TM structural units surrounding a pore. Its shape deforms the membrane locally into a dome. We present a hypothesis in which the membrane deformation changes upon channel opening. Quantitatively, membrane tension will alter gating energetics in proportion to the change in projected area under the dome. This mechanism can account for highly sensitive mechanical gating in the setting of a narrow, cation-selective pore.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Piezo2 in Cutaneous and Proprioceptive Mechanotransduction in Vertebrates.

Mechanosensitivity is a fundamental physiological capacity, which pertains to all life forms. Progress has been made with regard to understanding mechanosensitivity in bacteria, flies, and worms. In vertebrates, however, the molecular identity of mechanotransducers in somatic and neuronal cells has only started to appear. The Piezo family of mechanogated ion channels marks a pivotal milestone i...

متن کامل

Analysis of Dome Structure in Iranian - Islamic architecture and urban structure

The most important characteristic of dome and factors influencing it in the Islamic Iranian architecture that is different from other courses is construction and construction methods of them. Over time, different factors are involved in the process of formation of the domes. It is one of the factors influencing the structure of Iranian-Islamic domes. By examining the structure of Iranian-Islami...

متن کامل

Bio-inspired design of tactile sensors based on ionic polymer metal composites

SUMMARY Ionic polymer metal composites (IPMC) have been widely investigated as newly-emerging materials for transducer applications due to their good performance, such as low weight, good flexibility and large strain. A bio-inspired design was described and applied to build 3-D papillae dome structure for vectorial tactile sensors. INTRODUCTION The electrical-chemical-mechanical effect in ionic...

متن کامل

Zone of Transition in Seljuq Dome Chambers of Iran

Architecturally, the Seljuqs' dominion makes a significant shift from the Pre-Islamic Sassanid squinches into a sophisticated transition mechanism employed to change the walls of a square chamber to an octagonal base to set a dome which this initiated a new construction methodology to hybridize the previous experiences of Sassanid domes with new architectural tendencies since the previous under...

متن کامل

Mechanosensitivity of STREX-lacking BKCa channels in the colonic smooth muscle of the mouse.

Stretch sensitivity of Ca²(+)-activated large-conductance K(+) channels (BK(Ca)) has been observed in a variety of cell types and considered to be a potential mechanism in mechanoelectric transduction (MET). Mechanical stress is a major stimulator for the smooth muscle in the gastrointestinal (GI) tract. However, much about the role and mechanism of MET in GI smooth muscles remains unknown. The...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2017